BT-474

BT-474，亦稱ATCC HTB-20，是人類高度非整倍體乳腺導管癌細胞系^[1]，最初分離自一位60歲的白人女性乳腺浸潤性導管癌患者。BT-474細胞包含着許多具有典型凋亡細胞形態的細胞，已知細胞不存在參與凋亡誘導的p53蛋白，而抑制細胞凋亡的Bcl-2（英语：Bcl-2）則以中等水平表達。BT-474細胞傾向轉移到局部淋巴結，並且能夠在肺部形成微轉移病變^[2]。BT-474有55個染色體，並且具有極其複雜的基因組序列，大多數染色體計數在超四倍體的範圍內，同時又缺失三個染色體（N11、N13和N22），並且某些染色體（N9、N14和N15）存在着低於適當比例的問題^[3]。經過吉姆薩胰蛋白酶帶的測試後，未顯示出有明顯的HeLa標記物，並且在放射免疫分析中，沒有檢測到α-乳清蛋白的產生，但是發現BT-483細胞高度表達着孕酮受體^[4]。BT-474應用於研究癌症生物學和開發新藥物^[3]。

參考資料

^ Lasfargues, EY; Coutinho, WG; Dion, AS. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication.. In vitro. 1979-09, 15 (9): 723–9 [2019-12-07]. PMID 94035. doi:10.1007/bf02618252.
^ van Slooten, HJ; Bonsing, BA; Hiller, AJ; Colbern, GT; van Dierendonck, JH; Cornelisse, CJ; Smith, HS. Outgrowth of BT-474 human breast cancer cells in immune-deficient mice: a new in vivo model for hormone-dependent breast cancer.. British journal of cancer. 1995-07, 72 (1): 22–30 [2019-12-07]. PMID 7599056. doi:10.1038/bjc.1995.271.
^ ^3.0 ^3.1 Ciotlos, S; Mao, Q; Zhang, RY; Li, Z; Chin, R; Gulbahce, N; Liu, SJ; Drmanac, R; Peters, BA. Whole genome sequence analysis of BT-474 using complete Genomics' standard and long fragment read technologies.. GigaScience. 2016, 5: 8 [2019-12-07]. PMID 26865974. doi:10.1186/s13742-016-0113-x.
^ Lasfargues, EY; Coutinho, WG; Redfield, ES. Isolation of two human tumor epithelial cell lines from solid breast carcinomas.. Journal of the National Cancer Institute. 1978-10, 61 (4): 967–78 [2019-12-07]. PMID 212572. （原始内容存档于2013-12-17）.

外部連結

Cellosaurus entry for BT-474（页面存档备份，存于互联网档案馆）

[1] Lasfargues, EY; Coutinho, WG; Dion, AS. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication.. In vitro. 1979-09, 15 (9): 723–9 [2019-12-07]. PMID 94035. doi:10.1007/bf02618252.

[2] van Slooten, HJ; Bonsing, BA; Hiller, AJ; Colbern, GT; van Dierendonck, JH; Cornelisse, CJ; Smith, HS. Outgrowth of BT-474 human breast cancer cells in immune-deficient mice: a new in vivo model for hormone-dependent breast cancer.. British journal of cancer. 1995-07, 72 (1): 22–30 [2019-12-07]. PMID 7599056. doi:10.1038/bjc.1995.271.

[a-3] 3.0 ^3.1 Ciotlos, S; Mao, Q; Zhang, RY; Li, Z; Chin, R; Gulbahce, N; Liu, SJ; Drmanac, R; Peters, BA. Whole genome sequence analysis of BT-474 using complete Genomics' standard and long fragment read technologies.. GigaScience. 2016, 5: 8 [2019-12-07]. PMID 26865974. doi:10.1186/s13742-016-0113-x.

[4] Lasfargues, EY; Coutinho, WG; Redfield, ES. Isolation of two human tumor epithelial cell lines from solid breast carcinomas.. Journal of the National Cancer Institute. 1978-10, 61 (4): 967–78 [2019-12-07]. PMID 212572. （原始内容存档于2013-12-17）.

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