High Five細胞

High Five（正式名稱為BTI-Tn-5B1-4）是一種昆蟲細胞系，起源於粉紋夜蛾（英语：Cabbage looper）的卵巢細胞^[1]。它是由美國的植物研究所BTI（英语：Boyce Thompson Institute）發現的。High Five 細胞是使用桿狀病毒或轉染，進行重組蛋白表達的其中一個最常用細胞系，並且比Sf9細胞等鱗翅類昆蟲細胞系表達更多的重組蛋白^[2]^[3]^[4]。它們可以在沒有血清的情況下生長，並且可以以鬆散的附著狀態或懸濁液培養^[5] 。High Five細胞可產生大量的小分子核糖核酸、小分子干擾核糖核酸及piRNA，使其適合於研究以上三種類型的小沉默核糖核酸^[6]。

參考資料

^ Wickham, TJ; Davis, T; Granados, RR; Shuler, ML; Wood, HA. Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.. Biotechnology progress. NaN, 8 (5): 391–6 [2019-11-23]. PMID 1369220. doi:10.1021/bp00017a003. 请检查|date=中的日期值 (帮助)
^ Davis, TR; Trotter, KM; Granados, RR; Wood, HA. Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion.. Bio/technology (Nature Publishing Company). 1992-10, 10 (10): 1148–50 [2019-11-23]. PMID 1368794. doi:10.1038/nbt1092-1148.
^ Wickham, TJ; Nemerow, GR. Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system.. Biotechnology progress. NaN, 9 (1): 25–30 [2019-11-23]. PMID 7764044. doi:10.1021/bp00019a004. 请检查|date=中的日期值 (帮助)
^ Granados RR, Guoxun L, Derksen AC, McKenna KA. A New Insect Cell Line from Trichoplusia ni (BTI-Tn-5B1-4) Susceptible to Trichoplusia ni Single Enveloped Nuclear Polyhedrosis. Virus. J. Invertebr. Pathol. 1994, 64: 260–266. doi:10.1016/s0022-2011(94)90400-6.
^ Invitrogen. Cell Lines. Growth and Maintenance of Insect cell lines (PDF). A.0. Thermo Fisher Scientific. 31 March 2015 [2015-11-09]. （原始内容存档 (PDF)于2016-03-04）. |number=被忽略 (帮助)
^ Fu, Yu; Yang, Yujing; Zhang, Han; Farley, Gwen; Wang, Junling; Quarles, Kaycee A; Weng, Zhiping; Zamore, Phillip D. The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology. eLife. 2018-01-29, 7 [2019-11-23]. ISSN 2050-084X. PMC 5844692 . doi:10.7554/eLife.31628. （原始内容存档于2019-11-23）（英语）.

外部鏈接

Cellosaurus entry for High Five（页面存档备份，存于互联网档案馆）

[1] Wickham, TJ; Davis, T; Granados, RR; Shuler, ML; Wood, HA. Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.. Biotechnology progress. NaN, 8 (5): 391–6 [2019-11-23]. PMID 1369220. doi:10.1021/bp00017a003. 请检查|date=中的日期值 (帮助)

[2] Davis, TR; Trotter, KM; Granados, RR; Wood, HA. Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion.. Bio/technology (Nature Publishing Company). 1992-10, 10 (10): 1148–50 [2019-11-23]. PMID 1368794. doi:10.1038/nbt1092-1148.

[3] Wickham, TJ; Nemerow, GR. Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system.. Biotechnology progress. NaN, 9 (1): 25–30 [2019-11-23]. PMID 7764044. doi:10.1021/bp00019a004. 请检查|date=中的日期值 (帮助)

[4] Granados RR, Guoxun L, Derksen AC, McKenna KA. A New Insect Cell Line from Trichoplusia ni (BTI-Tn-5B1-4) Susceptible to Trichoplusia ni Single Enveloped Nuclear Polyhedrosis. Virus. J. Invertebr. Pathol. 1994, 64: 260–266. doi:10.1016/s0022-2011(94)90400-6.

[5] Invitrogen. Cell Lines. Growth and Maintenance of Insect cell lines (PDF). A.0. Thermo Fisher Scientific. 31 March 2015 [2015-11-09]. （原始内容存档 (PDF)于2016-03-04）. |number=被忽略 (帮助)

[6] Fu, Yu; Yang, Yujing; Zhang, Han; Farley, Gwen; Wang, Junling; Quarles, Kaycee A; Weng, Zhiping; Zamore, Phillip D. The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology. eLife. 2018-01-29, 7 [2019-11-23]. ISSN 2050-084X. PMC 5844692 . doi:10.7554/eLife.31628. （原始内容存档于2019-11-23）（英语）.

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