多重序列比对
![](http://images.weserv.nl/?url=//upload.wikimedia.org/wikipedia/commons/thumb/7/79/RPLP0_90_ClustalW_aln.gif/400px-RPLP0_90_ClustalW_aln.gif)
多重序列比对(Multiple sequence alignment;MSA)是对三个以上的生物学序列(biological sequence),如蛋白质序列、DNA序列或RNA序列所作的序列比对。一般来说,是输入一组假定拥有演化关系的序列。从MSA的结果可推导出序列的同源性,而种系发生关系也可引导出这些序列共同的演化始祖。如右图般的视觉化叙述可描绘出各种突变事件,例如点突变的单格变化,或是如删除突变与插入突变,可使各个序列之间产生鸿沟。MSA常用来研究序列的保守性,或是蛋白质结构域的三级结构与二级结构,甚至是个别的氨基酸或核苷酸。
参见
外部链接
- ExPASy sequence alignment tools (页面存档备份,存于互联网档案馆)
- Multiple Alignment Resource Page from the Virtual School of Natural Sciences
- Tools for Multiple Alignments from Pôle Bioinformatique Lyonnais
- Multiple sequence alignment lectures(页面存档备份,存于互联网档案馆) from the Max Planck Institute for Molecular Genetics
相关文献
- Duret, L.; S. Abdeddaim. Multiple alignment for structural functional or phylogenetic analyses of homologous sequences. D. Higgins and W. Taylor (编). Bioinformatics sequence structure and databanks. Oxford: Oxford University Press. 2000.
- Notredame, C. Recent progresses in multiple sequence alignment: a survey. Pharmacogenomics. 2002, 31 (1): 131 –– 144.
- Thompson, J. D.; F. Plewniak and O. Poch. A comprehensive comparison of multiple sequence alignment programs. Nucleic Acids Research. 1999, 27 (13): 12682––2690.
- Wallace, I.M.; Blackshields G and Higgins DG. Multiple sequence alignments. Curr Opin Struct Biol. 2005, 15 (3): 261–6.