COS-7
COS-7 (亦称ATCC CRL-1651、CV-1 in Origin Simian-7) 是在1980年代初期发现的非洲绿猴肾细胞系，最初是经带有编码野生型T抗原的起始失活突变的猿猴空泡病毒40转染而建立[1][2]，具有成纤维细胞样和病毒敏感性的独特特征[1][2]。稳定的细胞系是转运体相互作用研究及体外评价药物候选物的理想模型，其中常用的其中一个宿主细胞系就是COS-7细胞。COS-7细胞也会应用在细胞基因转录的研究及DNA质体转染实验[3]，从而分析类固醇生成基因[4]、类固醇受体的功能[5]及类固醇对功能分子的作用[6]。COS-7是源自非类固醇生成器官的非类固醇生成细胞[7][8]，然而有研究表明COS-7细胞可将3H-睾酮代谢为3H-雄烯二酮[9]。
参考资料
- ^ 1.0 1.1 Gluzman, Y. SV40-transformed simian cells support the replication of early SV40 mutants.. Cell. 1981-01, 23 (1): 175–82 [2019-12-08]. PMID 6260373. doi:10.1016/0092-8674(81)90282-8.
- ^ 2.0 2.1 Mellon, P; Parker, V; Gluzman, Y; Maniatis, T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.. Cell. 1981-12, 27 (2 Pt 1): 279–88 [2019-12-08]. PMID 6277501. doi:10.1016/0092-8674(81)90411-6.
- ^ Smale, ST; Baltimore, D. The "initiator" as a transcription control element.. Cell. 1989-04-07, 57 (1): 103–13 [2019-12-08]. PMID 2467742. doi:10.1016/0092-8674(89)90176-1.
- ^ Kawamoto, T; Mitsuuchi, Y; Toda, K; Yokoyama, Y; Miyahara, K; Miura, S; Ohnishi, T; Ichikawa, Y; Nakao, K; Imura, H. Role of steroid 11 beta-hydroxylase and steroid 18-hydroxylase in the biosynthesis of glucocorticoids and mineralocorticoids in humans.. Proceedings of the National Academy of Sciences of the United States of America. 1992-02-15, 89 (4): 1458–62 [2019-12-08]. PMID 1741400. doi:10.1073/pnas.89.4.1458.
- ^ Simental, JA; Sar, M; Lane, MV; French, FS; Wilson, EM. Transcriptional activation and nuclear targeting signals of the human androgen receptor.. The Journal of biological chemistry. 1991-01-05, 266 (1): 510–8 [2019-12-08]. PMID 1985913.
- ^ Topor, LS; Asai, M; Dunn, J; Majzoub, JA. Cortisol stimulates secretion of dehydroepiandrosterone in human adrenocortical cells through inhibition of 3betaHSD2.. The Journal of clinical endocrinology and metabolism. 2011-01, 96 (1): E31–9 [2019-12-08]. PMID 20943790. doi:10.1210/jc.2010-0692.
- ^ West, LA; Horvat, RD; Roess, DA; Barisas, BG; Juengel, JL; Niswender, GD. Steroidogenic acute regulatory protein and peripheral-type benzodiazepine receptor associate at the mitochondrial membrane.. Endocrinology. 2001-01, 142 (1): 502–5 [2019-12-08]. PMID 11145616. doi:10.1210/endo.142.1.8052.
- ^ Rasheeda, MK; Kagawa, H; Kirubagaran, R; Dutta-Gupta, A; Senthilkumaran, B. Cloning, expression and enzyme activity analysis of testicular 11beta-hydroxysteroid dehydrogenase during seasonal cycle and after hCG induction in air-breathing catfish Clarias gariepinus.. The Journal of steroid biochemistry and molecular biology. 2010-05, 120 (1): 1–10 [2019-12-08]. PMID 20219674. doi:10.1016/j.jsbmb.2010.02.014.
- ^ Nozaki, M; Haraguchi, S; Miyazaki, T; Shigeta, D; Kano, N; Lei, XF; Kim-Kaneyama, JR; Minakata, H; Miyazaki, A; Tsutsui, K. Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.. Scientific reports. 2018-02-01, 8 (1): 2167 [2019-12-08]. PMID 29391479. doi:10.1038/s41598-018-20226-2.