COS-7
COS-7 (亦稱ATCC CRL-1651、CV-1 in Origin Simian-7) 是在1980年代初期發現的非洲綠猴腎細胞系，最初是經帶有編碼野生型T抗原的起始失活突變的猿猴空泡病毒40轉染而建立[1][2]，具有成纖維細胞樣和病毒敏感性的獨特特徵[1][2]。穩定的細胞系是轉運體相互作用研究及體外評價藥物候選物的理想模型，其中常用的其中一個宿主細胞系就是COS-7細胞。COS-7細胞也會應用在細胞基因轉錄的研究及DNA質體轉染實驗[3]，從而分析類固醇生成基因[4]、類固醇受體的功能[5]及類固醇對功能分子的作用[6]。COS-7是源自非類固醇生成器官的非類固醇生成細胞[7][8]，然而有研究表明COS-7細胞可將3H-睪酮代謝為3H-雄烯二酮[9]。
參考資料
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- ^ 2.0 2.1 Mellon, P; Parker, V; Gluzman, Y; Maniatis, T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.. Cell. 1981-12, 27 (2 Pt 1): 279–88 [2019-12-08]. PMID 6277501. doi:10.1016/0092-8674(81)90411-6.
- ^ Smale, ST; Baltimore, D. The "initiator" as a transcription control element.. Cell. 1989-04-07, 57 (1): 103–13 [2019-12-08]. PMID 2467742. doi:10.1016/0092-8674(89)90176-1.
- ^ Kawamoto, T; Mitsuuchi, Y; Toda, K; Yokoyama, Y; Miyahara, K; Miura, S; Ohnishi, T; Ichikawa, Y; Nakao, K; Imura, H. Role of steroid 11 beta-hydroxylase and steroid 18-hydroxylase in the biosynthesis of glucocorticoids and mineralocorticoids in humans.. Proceedings of the National Academy of Sciences of the United States of America. 1992-02-15, 89 (4): 1458–62 [2019-12-08]. PMID 1741400. doi:10.1073/pnas.89.4.1458.
- ^ Simental, JA; Sar, M; Lane, MV; French, FS; Wilson, EM. Transcriptional activation and nuclear targeting signals of the human androgen receptor.. The Journal of biological chemistry. 1991-01-05, 266 (1): 510–8 [2019-12-08]. PMID 1985913.
- ^ Topor, LS; Asai, M; Dunn, J; Majzoub, JA. Cortisol stimulates secretion of dehydroepiandrosterone in human adrenocortical cells through inhibition of 3betaHSD2.. The Journal of clinical endocrinology and metabolism. 2011-01, 96 (1): E31–9 [2019-12-08]. PMID 20943790. doi:10.1210/jc.2010-0692.
- ^ West, LA; Horvat, RD; Roess, DA; Barisas, BG; Juengel, JL; Niswender, GD. Steroidogenic acute regulatory protein and peripheral-type benzodiazepine receptor associate at the mitochondrial membrane.. Endocrinology. 2001-01, 142 (1): 502–5 [2019-12-08]. PMID 11145616. doi:10.1210/endo.142.1.8052.
- ^ Rasheeda, MK; Kagawa, H; Kirubagaran, R; Dutta-Gupta, A; Senthilkumaran, B. Cloning, expression and enzyme activity analysis of testicular 11beta-hydroxysteroid dehydrogenase during seasonal cycle and after hCG induction in air-breathing catfish Clarias gariepinus.. The Journal of steroid biochemistry and molecular biology. 2010-05, 120 (1): 1–10 [2019-12-08]. PMID 20219674. doi:10.1016/j.jsbmb.2010.02.014.
- ^ Nozaki, M; Haraguchi, S; Miyazaki, T; Shigeta, D; Kano, N; Lei, XF; Kim-Kaneyama, JR; Minakata, H; Miyazaki, A; Tsutsui, K. Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.. Scientific reports. 2018-02-01, 8 (1): 2167 [2019-12-08]. PMID 29391479. doi:10.1038/s41598-018-20226-2.